N Recombinant Monoclonal Antibody, FITC conjugated

Code CSB-RA33255C1GMY
Size US$210
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Product Details

Uniprot No.
Target Names
N (Nucleoprotein)
Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Conjugate
FITC
Clonality
Monoclonal
Isotype
Mouse scFv fusion with human IgG1 Fc
Clone No.
1A6
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The creation of the N recombinant monoclonal antibody follows a rigorous process to ensure its quality and specificity. It begins with the isolation of B cells from the spleen of an immunized animal, using the recombinant human SARS-CoV-2 N protein (1-419aa) as the immunogen. The RNA is extracted from these B cells and converted into cDNA through reverse transcription. The N antibody genes are then amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. The human IgG1 Fc is integrated into the vector, downstream of the N antibody gene and the FITC is also inserted into the vector. This recombinant vector is transfected into host cells, allowing for the production of the N recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and subjected to purification using affinity chromatography, resulting in a highly purified form. The purified N recombinant monoclonal antibody can be used to detect human SARS-CoV-2 N protein. Through this meticulous production process, a reliable and effective N recombinant monoclonal antibody is obtained, which serves as a valuable tool in various research related to SARS-CoV-2.

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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