Recombinant Helicobacter pylori Urease subunit beta (ureB)

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Code CSB-EP300910HUV
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
ureB
Uniprot No.
Alternative Names
HP_0072; hpuB; URE1_HELPY; Urea amidohydrolase subunit beta; Urease subunit beta; ureB
Species
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Source
E.coli
Expression Region
1-569aa
Target Protein Sequence
MKKISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSNNPSKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEALAGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKWMLRAAEEYSMNLGFLAKGNASNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKYDVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTNPTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSSDSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISEYVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAHHGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCRNITKKDMQFNDTTAHIEVNPETYHVFVDGKEVTSKPANKVSLAQLFSIF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
65.7kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant H. pylori ureB protein is encoded by the gene of ureB (1-569aa). The gene of ureB was cloned in a system (E.coli) that supported the expression of ureB and translation of messenger RNA. Modification of ureB by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 6xHis tag in the production. The purity is 90% determined by SDS-PAGE.

UreB is a protein coding gene that encodes Urease subunit beta. According to some studies, UreB may have the following features.
The ureA and ureB genes encoding H. pylori urease are transcribed and translated in E. coli, and these genes alone are sufficient to synthesize and assemble the bulk-sized enzyme. The basic properties of H. pylori urease activity on colonization indicate that H. pylori urease is an excellent reporter of gene expression in H. pylori. Recombinant L. lactis expressing UreB-IL-2 has the potential to be used as an edible vaccine for the control of Helicobacter pylori infection. UreB transgenic carrots have the potential to be used as an edible vaccine for the control of Helicobacter pylori. UreB enhances the stability of UreC against proteolysis.

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Target Background

Function
Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.
Gene References into Functions
  1. Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma. PMID: 26214524
Subcellular Location
Cytoplasm. Note=Also associates with the outer membrane upon autolysis of neighboring bacteria.
Protein Families
Metallo-dependent hydrolases superfamily, Urease alpha subunit family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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